| Reagent 1 | 1 x 30 ml |
| Reagent 2 | 1 x 8 ml |
| Standard | 1 x 4 ml |
| Quality Control - Level 1 | 1 x 4 ml |
| Quality Control - Level 2 | 1 x 4 ml |
Zinc Assay Kit
Zinc is the second most abundant trace element in the body, found in more than 300 metalloenzymes. Carbonic anhydrase, alkaline phosphatase, DNA and RNA polymerases could be listed as important examples. Due to its multiple biochemical functions, zinc deficiency manifests by variety of conditions. Growth failure and stunning, alterations in immune function, diarrhea, skin lesions and alopecia are observed in cases of clinical deficiency. Subclinical deficiency has effects on immune function, synthesis and action of hormones, and neurological function
Zinc is the second most abundant trace element in the body, found in more than 300 metalloenzymes. Carbonic anhydrase, alkaline phosphatase, DNA and RNA polymerases could be listed as important examples. Due to its multiple biochemical functions, zinc deficiency manifests by variety of conditions. Growth failure and stunning, alterations in immune function, diarrhea, skin lesions and alopecia are observed in cases of clinical deficiency. Subclinical deficiency has effects on immune function, synthesis and action of hormones, and neurological function.
Zinc found in the samples change the red-orange color
of 5-Br-PAPS to light pink under alkaline conditions. The change of absorbance
at 548 nm is proportional to total zinc level in the sample. The assay can be
calibrated with zinc sulfate dissolved in deionized water.
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2. Rink L, Gabriel
P. Zinc and the Immune System. Proc Nutr Soc. 2000;59:541-52
3. Homsher R, Zak
B. Spectrophotometric Investigation of Sensitive Complexing Agents for the
Determination of Zinc in Serum. Clin Chem. 1985;31(8):1310-3.
4. Barnes PM,
Moynahan EJ. Zinc Deficiency in Acrodermatitis Enteropathica: Multiple Dietary
Ontolerance Treated with Synthetic Diet. Proc roy Soc Med. 1973;66:327-9.
5. Prasad AS. Zinc
Deficiency. BMJ. 2003;326:409-10.
6. Young DS.
Effects of disease on Clinical Lab. Tests, 4th ed AACC 2001.
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