3. Generation Total Antioxidant Status

FILES


- Package Insert Of TAS Assay Kit.pdf

- RelatedArticles.pdf

 

Summary and Explanation
Reactive oxygen species (ROS) is produced in metabolic
and physiological processes, and harmful oxidative reactions may
occur in organisms that remove them via enzymatic and nonenzymatic
antioxidative mechanisms. Under certain conditions, the
increase in oxidants and decrease in antioxidants cannot be
prevented, and the oxidative or in over 100 disorders, develops.


Antioxidant molecules prevent or inhibit these harmful
reactions2. Serum (or plasma) concentrations of different
antioxidants can be measured in laboratories separately, but the
measurements are time-consuming, labor-intensive, costly, and
require complicated techniques. Because the measurement
measurement of different antioxidant molecules separately is not
practical and their antioxidant effects are additive, the total
antioxidant capacity of a sample is measured, and this is called
total antioxidant capacity (TAC) 3, total antioxidant activity (TAA) 4,
total antioxidant power (TAOP) 5, total antioxidant status (TAS) 6,
total antioxidant response 7, or other synonyms.


Principle of Assay
Antioxidants in the sample reduce dark blue-green
colored ABTS radical to colorless reduced ABTS form. The change
of absorbance at 660 nm is related with total antioxidant level of
the sample. The assay is calibrated with a stable antioxidant
standard solution which is traditionally named as Trolox Equivalent
that is a vitamin E analog .


Components
All reagents and standards are ready to use.

  • Reagent 1 (Assay Buffer) 1 x 50 ml
  • Reagent 2 (Colored ABTS Radical Solution) 1 x 10 ml
  • *Standard 1 (0.0 mmolTrolox Equiv./L) Solution(Not included)
  • Standard 2 (1.0 mmolTrolox Equiv./L) Solution1 x 10 ml

*You should use any deionised-water


Storage Conditions
This kit should be stored at 4oC.
Additional Items Required
A spectrophotometer or a plate reader or an automated
biochemistry analyzer.


Samples:
Blood serum, plasma, semen plasma, saliva, urine, cell lysates,
tissue homogenates, beverages, fruit juices and oils (oils require
different reagent 1) can be used as sample.



 
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